Peptide-coated semiconductor nanocrystals for biomedical applications

Xavier Michalet; Fabien F. Pinaud; Laurent A. Bentolila; James M. Tsay; Soeren Doose; Jianqing J. Li; G. Iyer; Shimon Weiss

doi:10.1117/12.589498

4 April 2005 Peptide-coated semiconductor nanocrystals for biomedical applications

Xavier Michalet, Fabien F. Pinaud, Laurent A. Bentolila, James M. Tsay, Soeren Doose, Jianqing J. Li, G. Iyer, Shimon Weiss

Author Affiliations +

Proceedings Volume 5704, Genetically Engineered and Optical Probes for Biomedical Applications III; (2005) https://doi.org/10.1117/12.589498
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have developed a new functionalization approach for semiconductor nanocrystals based on a single-step exchange of surface ligands with custom-designed peptides. This peptide-coating technique yield small, monodisperse and very stable water-soluble NCs that remain bright and photostable. We have used this approach on several types of core and core-shell NCs in the visible and near-infrared spectrum range and used fluorescence correlation spectroscopy for rapid assessment of the colloidal and photophysical properties of the resulting particles. This peptide coating strategy has several advantages: it yields probes that are immediately biocompatible; it is amenable to improvements of the different properties (solubilization, functionalization, etc) via rational design, parallel synthesis, or molecular evolution; it permits the combination of several functions on individual NCs. These functionalized NCs have been used for diverse biomedical applications. Two are discussed here: single-particle tracking of membrane receptor in live cells and combined fluorescence and PET imaging of targeted delivery in live animals.

Citation Download Citation

Xavier Michalet, Fabien F. Pinaud, Laurent A. Bentolila, James M. Tsay, Soeren Doose, Jianqing J. Li, G. Iyer, and Shimon Weiss "Peptide-coated semiconductor nanocrystals for biomedical applications", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); https://doi.org/10.1117/12.589498

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