Synthesis and characterization of CdTe nanocrystals for applications as biolabels

Wendel L. Moreira; Adriana Fontes; Andre Thomaz; Antonio A.R. Neves; Luiz Carlos Barbosa; Frederico D. de Menezes; Patricia M. A. de Farias; Beate S. Santos; Carlos Lenz Cesar

doi:10.1117/12.590841

4 April 2005 Synthesis and characterization of CdTe nanocrystals for applications as biolabels

Wendel L. Moreira, Adriana Fontes, Andre Thomaz, Antonio A.R. Neves, Luiz Carlos Barbosa, Frederico D. de Menezes, Patricia M. A. de Farias, Beate S. Santos, Carlos Lenz Cesar

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Proceedings Volume 5704, Genetically Engineered and Optical Probes for Biomedical Applications III; (2005) https://doi.org/10.1117/12.590841
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Semiconductor nanocrystals composed by few hundred to a few thousand atoms also known as quantum dots have received substantial attention due to their size tunable narrow-emission spectra and several other advantages over organic molecules as fluorescent labels for biological applications, including resistence to photodegradation, improved brightness and only one laser excitation that enable the monitoring of several processes simultaneously. In this work we have synthesized and characterized thiol-capped CdTe and bioconjugated them to macrophages. We have mapped the fluoroscence intensity along the macrophage's body in our set up consisting of an optical tweezer plus a non-linear micro-spectroscopy system to perform scanning microscopy and observe spectra using two photon excited luminescence.

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Wendel L. Moreira, Adriana Fontes, Andre Thomaz, Antonio A.R. Neves, Luiz Carlos Barbosa, Frederico D. de Menezes, Patricia M. A. de Farias, Beate S. Santos, and Carlos Lenz Cesar "Synthesis and characterization of CdTe nanocrystals for applications as biolabels", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); https://doi.org/10.1117/12.590841

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KEYWORDS

Luminescence

Nanocrystals

Quantum dots

Confocal microscopy

Fluorescent markers

Microscopes

Microscopy

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