In vivo investigation of protein adsorption on implant surfaces

Reiner Salzer; Gerald Steiner; Sibel Tunc

doi:10.1117/12.598386

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9 May 2005 In vivo investigation of protein adsorption on implant surfaces

Reiner Salzer, Gerald Steiner, Sibel Tunc

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Proceedings Volume 5768, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems IV; (2005) https://doi.org/10.1117/12.598386
Event: Nondestructive Evaluation for Health Monitoring and Diagnostics, 2005, San Diego, CA, United States

Abstract

A decisive problem in biomedical or biomaterial research of the post-genomic era is the determination of protein structure and function. Common techniques that can give full structural information do not permit in-vivo measurements. Vibrational Proteomics, an innovative combination of biochemical techniques and infrared or Raman spectroscopy, can provide information which will help substantially to fill this gap. Infrared and Raman spectroscopy are well established as methods for qualitative and quantitative analysis of protein secondary structure, in solution and even when adsorbed to implant surfaces. Their singular advantage over other techniques is that spectra can be obtained for proteins in a wide range of environments, in solutions and on surfaces including polymers, metals and bioceramics. Here we report on structural changes in fibrinogen from the dissolved to the adsorbed state on implant material of different hydrophobicity. FTIR imaging permits the identification of coagulation spots on the implant.

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Reiner Salzer, Gerald Steiner, and Sibel Tunc "In vivo investigation of protein adsorption on implant surfaces", Proc. SPIE 5768, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems IV, (9 May 2005); https://doi.org/10.1117/12.598386

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