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9 May 2005 In vivo investigation of protein adsorption on implant surfaces
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A decisive problem in biomedical or biomaterial research of the post-genomic era is the determination of protein structure and function. Common techniques that can give full structural information do not permit in-vivo measurements. Vibrational Proteomics, an innovative combination of biochemical techniques and infrared or Raman spectroscopy, can provide information which will help substantially to fill this gap. Infrared and Raman spectroscopy are well established as methods for qualitative and quantitative analysis of protein secondary structure, in solution and even when adsorbed to implant surfaces. Their singular advantage over other techniques is that spectra can be obtained for proteins in a wide range of environments, in solutions and on surfaces including polymers, metals and bioceramics. Here we report on structural changes in fibrinogen from the dissolved to the adsorbed state on implant material of different hydrophobicity. FTIR imaging permits the identification of coagulation spots on the implant.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Reiner Salzer, Gerald Steiner, and Sibel Tunc "In vivo investigation of protein adsorption on implant surfaces", Proc. SPIE 5768, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems IV, (9 May 2005);

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