The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

Eoghan O Faolain; Mary B. Hunter; Joe M. Byrne; Peter Kelehan; Hugh J. Byrne; Fiona M. Lyng

doi:10.1117/12.603344

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3 June 2005 The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

Eoghan O Faolain, Mary B. Hunter, Joe M. Byrne, Peter Kelehan, Hugh J. Byrne, Fiona M. Lyng

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Proceedings Volume 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy; (2005) https://doi.org/10.1117/12.603344
Event: OPTO-Ireland, 2005, Dublin, Ireland

Abstract

The application of vibrational spectroscopy to disease diagnosis is a relatively new, rapidly evolving scientific field. Techniques such as Raman and infrared spectroscopy have shown great promise in this regard over the past number of years. This study directly compared Raman spectroscopy and synchrotron infrared (SR-IR) spectroscopy on parallel cervical cancer samples. Both frozen and dewaxed formalin fixed paraffin preserved tissue sections were examined. Both tissue types produced good quality Raman and SR-IR spectra, although the lesser processed, frozen tissue sections displayed the most detailed spectra. Spectroscopy was shown capable of discriminating between different cell types in normal cervical tissue. Spectra recorded from invasive carcinoma showed a marked difference from those recorded from normal cervical epithelial cells. Spectral differences identified with the onset of carcinogenesis include increased nucleic acid contributions and decreased glycogen levels. These investigations pave the way for an enlarged study into this exciting new diagnostic field.

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Eoghan O Faolain, Mary B. Hunter, Joe M. Byrne, Peter Kelehan, Hugh J. Byrne, and Fiona M. Lyng "The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field", Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); https://doi.org/10.1117/12.603344

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