Optics-based sensor for DNA screening applications

Wei Ming Chiang; Chu Sing Lim; Tet Fatt Chia; Wai Fun Tan; Seng Kim Lim

doi:10.1117/12.621180

12 April 2005 Optics-based sensor for DNA screening applications

Wei Ming Chiang, Chu Sing Lim, Tet Fatt Chia, Wai Fun Tan, Seng Kim Lim

Proceedings Volume 5852, Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics; (2005) https://doi.org/10.1117/12.621180
Event: Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, 2004, -, Singapore

Abstract

Short Tandem Repeats (STRs) are non-coding regions in genomic DNA that consist of short segments of core tandem repeat sequences. As these sequences are highly polymorphic in nature, they are extremely specific and unique to the individual. The STR fragments are amplified through the Polymerase Chain Reaction (PCR) before they are separated and analyzed. In this study, an optic-based system was developed to detect two STR loci in a gel matrix. Results show that the system is able to detect the STR fragments with a precision of +/- 1.0 bp, which is considered acceptable, given that the chosen STR loci have 4 bp repeat sequences. This system offers an inexpensive yet efficient method of STR DNA typing.

Citation Download Citation

Wei Ming Chiang, Chu Sing Lim, Tet Fatt Chia, Wai Fun Tan, and Seng Kim Lim "Optics-based sensor for DNA screening applications", Proc. SPIE 5852, Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, (12 April 2005); https://doi.org/10.1117/12.621180

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KEYWORDS

DNA profiling

Optical fibers

Polishing

Sensors

Forensic science

Blood

Health sciences

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