Apoptosis induces temporal increase in attenuation as measured by optical coherence tomography

Freek J van der Meer; Dirk J. Faber; Maurice C.G. Aalders; Jop Perree; Ton G. van Leeuwen

doi:10.1117/12.632992

7 October 2005 Apoptosis induces temporal increase in attenuation as measured by optical coherence tomography

Freek J van der Meer, Dirk J. Faber, Maurice C.G. Aalders, Jop Perree, Ton G. van Leeuwen

Proceedings Volume 5861, Optical Coherence Tomography and Coherence Techniques II; 58610Y (2005) https://doi.org/10.1117/12.632992
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis was induced. We analyzed the OCT data including both the scattering properties of the medium and the axial point spread function of the OCT system. We measured that the optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm^-1 to 1.3 ± 0.6 mm^-1, whereas with the apoptotic cells a clear increase (up to 6.4 ± 1.7 mm^-1) in scattering was observed. The results on cultured cells a presented in this study indicate the ability of OCT to detect and differentiate between viable, apoptotic and necrotic cells based on their backscatter properties. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on line monitoring of tissues, e.g. for feedback in cancer treatment.

Citation Download Citation

Freek J van der Meer, Dirk J. Faber, Maurice C.G. Aalders, Jop Perree, and Ton G. van Leeuwen "Apoptosis induces temporal increase in attenuation as measured by optical coherence tomography", Proc. SPIE 5861, Optical Coherence Tomography and Coherence Techniques II, 58610Y (7 October 2005); https://doi.org/10.1117/12.632992

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available