Antimicrobial photodynamic treatment of gram-negative bacteria with a cationic phenothiazine dye under pulsed light irradiation

Satoko Kawauchi; Shunichi Sato; Toru Yamaguchi; Nariyoshi Shinomiya; Daizo Saito; Hiroshi Ashida; Minoru Obara; Makoto Kikuchi

doi:10.1117/12.633093

26 August 2005 Antimicrobial photodynamic treatment of gram-negative bacteria with a cationic phenothiazine dye under pulsed light irradiation

Satoko Kawauchi, Shunichi Sato, Toru Yamaguchi, Nariyoshi Shinomiya, Daizo Saito, Hiroshi Ashida, Minoru Obara, Makoto Kikuchi

Author Affiliations +

Proceedings Volume 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II; 58630W (2005) https://doi.org/10.1117/12.633093
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

In-vitro photodynamic inactivation of Ps. aeruginosa with methylene blue under pulsed light excitation was investigated at different pulse repetition rates. Bacterial suspensions were illuminated with 670-nm nanosecond pulsed light with a peak intensity of 2.0 MW/cm² at pulse repetition rates in the range of 5-30 Hz. Photobactericidal effect increased with increasing pulse repetition rate for the same total light dose; more than two orders in magnitude reduction of bacterial survival fraction was obtained at 30 Hz. Such a positive dependence of photobactericidal effect on pulse repetition rate was inconsistent with our previous results for human lung cancer cells that were photodynamically treated with a lysosomal sensitizer. The reason for the increased photobactericidal effect at the high pulse repetition rate is discussed.

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Satoko Kawauchi, Shunichi Sato, Toru Yamaguchi, Nariyoshi Shinomiya, Daizo Saito, Hiroshi Ashida, Minoru Obara, and Makoto Kikuchi "Antimicrobial photodynamic treatment of gram-negative bacteria with a cationic phenothiazine dye under pulsed light irradiation", Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630W (26 August 2005); https://doi.org/10.1117/12.633093

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