Wavelet-based synchronization of nongated confocal microscopy data for 4D imaging of the embryonic heart

Michael Liebling; Arian S. Forouhar; Morteza Gharib; Scott E. Fraser; Mary E. Dickinson

doi:10.1117/12.615232

17 September 2005 Wavelet-based synchronization of nongated confocal microscopy data for 4D imaging of the embryonic heart

Michael Liebling, Arian S. Forouhar, Morteza Gharib, Scott E. Fraser, Mary E. Dickinson

Author Affiliations +

Proceedings Volume 5914, Wavelets XI; 591409 (2005) https://doi.org/10.1117/12.615232
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

With the availability of new confocal laser scanning microscopes, fast biological processes, such as the blood flow in living organisms at early stages of the embryonic development, can be observed with unprecedented time resolution. When the object under study has a periodic motion, e.g. a beating embryonic heart, the imaging capabilities can be extended to retrieve 4D data. We acquire nongated slice-sequences at increasing depth and retrospectively synchronize them to build dynamic 3D volumes. Here, we present a synchronization procedure based on the temporal correlation of wavelet features. The method is designed to handle large data sets and to minimize the influence of artifacts that are frequent in fluorescence imaging techniques such as bleaching, nonuniform contrast, and photon-related noise.

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Michael Liebling, Arian S. Forouhar, Morteza Gharib, Scott E. Fraser, and Mary E. Dickinson "Wavelet-based synchronization of nongated confocal microscopy data for 4D imaging of the embryonic heart", Proc. SPIE 5914, Wavelets XI, 591409 (17 September 2005); https://doi.org/10.1117/12.615232

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