Image Processing Of Muscle Striations Below The Resolution Limit Of The Light Microscope

David H. Burns; Dale N. Holdren; Ammasi Periasamy; William C. Everts; Gerald H. Pollack

doi:10.1117/12.952144

5 May 1986 Image Processing Of Muscle Striations Below The Resolution Limit Of The Light Microscope

David H. Burns, Dale N. Holdren, Ammasi Periasamy, William C. Everts, Gerald H. Pollack

Proceedings Volume 0593, Medical Image Processing; (1986) https://doi.org/10.1117/12.952144
Event: 1985 International Technical Symposium/Europe, 1985, Cannes, France

Abstract

We describe the use of digital deconvolution in the study of muscle striations below the resolution imposed by the optical diffraction of our video light microscope. To use deconvolution procedures on muscle images, the transfer function of the optical system is first characterized. This is accomplished by imaging a step object and fitting the image with the combination of a first order Bessel and Gaussian function using a non-linear least squares approach. Due to the ill-conditioned nature of deconvolution, however, ambiguity in the reconstruction is sometimes found. To allow better estimation of the true object, separate deconvolution approaches are used and the reconstructions compared. In this manner, the fine structure of muscle striations is determined.

Citation Download Citation

David H. Burns, Dale N. Holdren, Ammasi Periasamy, William C. Everts, and Gerald H. Pollack "Image Processing Of Muscle Striations Below The Resolution Limit Of The Light Microscope", Proc. SPIE 0593, Medical Image Processing, (5 May 1986); https://doi.org/10.1117/12.952144

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available