CARS imaging with a single laser pulse

Christoph Heinrich; Stefan Bernet; Monika Ritsch-Marte

doi:10.1117/12.622802

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23 September 2005 CARS imaging with a single laser pulse

Christoph Heinrich, Stefan Bernet, Monika Ritsch-Marte

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Proceedings Volume 5959, Medical Imaging; 595902 (2005) https://doi.org/10.1117/12.622802
Event: Congress on Optics and Optoelectronics, 2005, Warsaw, Poland

Abstract

We report coherent anti-Stokes Raman scattering (CARS) microscopy with ns-pulses. The chosen wide-field geometry allows imaging of the whole field of view at once, without scanning of the sample. Tuning the difference of the two incident laser frequencies overlapping at the sample to a specific vibrational level, one can map the spatial distribution of selected Raman active molecules. Both the CARS signal of the surrounding solvent can be excited (negative contrast) as well as the signal of the structure embedded by the solvent (positive contrast). As a biological sample we used slices of a sunflower seed and tuned to the vibrational transition of its ingredient - linoleic acid - at 2870 cm^-1 which corresponds to the strongest C-H stretching vibration. Even with a single pair of laser pulses of 3 ns duration it was possible to acquire a rough, but still meaningful image.

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Christoph Heinrich, Stefan Bernet, and Monika Ritsch-Marte "CARS imaging with a single laser pulse", Proc. SPIE 5959, Medical Imaging, 595902 (23 September 2005); https://doi.org/10.1117/12.622802

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