Multi-modal miniature microscope - 4M device for bio-imaging applications: an overview of the system

Tomasz S. Tkaczyk; Jeremy D. Rogers; Mohammed Rahman; Todd C. Christenson; Stephen Gaalema; Eustace L. Dereniak; Rebecca Richards-Kortum; Michael R. Descour

doi:10.1117/12.626169

24 September 2005 Multi-modal miniature microscope: 4M Device for bio-imaging applications - an overview of the system

Tomasz S. Tkaczyk, Jeremy D. Rogers, Mohammed Rahman, Todd C. Christenson, Stephen Gaalema, Eustace L. Dereniak, Rebecca Richards-Kortum, Michael R. Descour

Author Affiliations +

Proceedings Volume 5959, Medical Imaging; 59590N (2005) https://doi.org/10.1117/12.626169
Event: Congress on Optics and Optoelectronics, 2005, Warsaw, Poland

Abstract

The multi-modal miniature microscope (4M) device to image morphology and cytochemistry in vivo is a microscope on a chip including optical, micro-mechanical, and electronic components. This paper describes all major system components: optical system, custom high speed CMOS detector and comb drive actuator. The hybrid sol-gel lenses, their fabrication and assembling technology, optical system parameters, and various operation modes (fluorescence, reflectance, structured illumination) are also discussed. A particularly interesting method is a structured illumination technique that delivers confocal-imaging capabilities and may be used for optical sectioning. For reconstruction of the sectioned layer a sine approximation algorithm is applied. Structured illumination is produced with LIGA fabricated actuator scanning in resonance. The spatial resolution of the system is 1 μm, and was magnified by 4x matching the CMOS pixel size of 4 μm (a lateral magnification is 4:1), and the extent of field of the system is 250μm. An overview of the 4M device is combined with the presentation of imaging results for epithelial cell phantoms with optical properties characteristic of normal and cancerous tissue labeled with nanoparticles.

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Tomasz S. Tkaczyk, Jeremy D. Rogers, Mohammed Rahman, Todd C. Christenson, Stephen Gaalema, Eustace L. Dereniak, Rebecca Richards-Kortum, and Michael R. Descour "Multi-modal miniature microscope: 4M Device for bio-imaging applications - an overview of the system", Proc. SPIE 5959, Medical Imaging, 59590N (24 September 2005); https://doi.org/10.1117/12.626169

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