Model for monitoring the process of photodynamic therapy in patients

Takato O. Yoshida; Eiji Kohno; Takashi Sakurai; Toru Hirano; Seiji Yamamoto M.D.; Susumu Terakawa M.D.

doi:10.1117/12.639221

11 September 2006 Model for monitoring the process of photodynamic therapy in patients

Takato O. Yoshida, Eiji Kohno, Takashi Sakurai, Toru Hirano, Seiji Yamamoto M.D., Susumu Terakawa M.D.

Proceedings Volume 5967, 2004 Shanghai International Conference on Laser Medicine and Surgery; 59670W (2006) https://doi.org/10.1117/12.639221
Event: 2004 Shanghai international Conference on Laser Medicine and Surgery, 2004, Shanghai, China

Abstract

The photodynamic therapy (PDT) on tumors is quite effective and widely applied but usually carried out without an immediate evaluation of results. We measured the tumor fluorescence in mice with a fiber probe connected to a linear array spectral analyzer (PMA-11, Hamamatsu Photonics). The spectrum showed a transient change in fluorescence color from red to green during Photofrin-mediated PDT. In order to examine the source of green fluorescence, the mitochondria were accessed under a Nipkow disk-scanning confocal microscope in the HeLa cell in culture after labeling them with a red fluorescent protein (DsRed1-mito) and staining the cell with Photofrin (Axcan Scandipharm). Changes in fluorescence color from red to green were observed in the area of mitochondria upon their swelling during irradiation. This finding in vitro provided clear evidence that the change in fluorescence color from red to green observed in vivo was due to the mitochondrial destruction associated with the cell-death by PDT. This technique of spectral monitoring in tumor may be useful for detection of the cell-death signal during PDT in patients.

Citation Download Citation

Takato O. Yoshida, Eiji Kohno, Takashi Sakurai, Toru Hirano, Seiji Yamamoto M.D., and Susumu Terakawa M.D. "Model for monitoring the process of photodynamic therapy in patients", Proc. SPIE 5967, 2004 Shanghai International Conference on Laser Medicine and Surgery, 59670W (11 September 2006); https://doi.org/10.1117/12.639221

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