Flow through PCR module of BioBriefcase

E. Arroyo; E. K. Wheeler; R. Shediac; B. Hindson; S. Nasarabadi; G. Vrankovich; P. Bell; C. Bailey; T. Sheppod; A. T. Christian

doi:10.1117/12.630844

11 November 2005 Flow through PCR module of BioBriefcase

E. Arroyo, E. K. Wheeler, R. Shediac, B. Hindson, S. Nasarabadi, G. Vrankovich, P. Bell, C. Bailey, T. Sheppod, A. T. Christian

Author Affiliations +

Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 600714 (2005) https://doi.org/10.1117/12.630844
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed with a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.

Citation Download Citation

E. Arroyo, E. K. Wheeler, R. Shediac, B. Hindson, S. Nasarabadi, G. Vrankovich, P. Bell, C. Bailey, T. Sheppod, and A. T. Christian "Flow through PCR module of BioBriefcase", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600714 (11 November 2005); https://doi.org/10.1117/12.630844

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