Use of optimization algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S. P. Poland; D. Burns; W. Lubeigt; B. A. Patterson; G. Valentine; A. J. Wright; J. M. Girkin

doi:10.1117/12.669388

8 June 2006 Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S. P. Poland, D. Burns, W. Lubeigt, B. A. Patterson, G. Valentine, A. J. Wright, J. M. Girkin

Author Affiliations +

Proceedings Volume 6018, 5th International Workshop on Adaptive Optics for Industry and Medicine; 60181H (2006) https://doi.org/10.1117/12.669388
Event: 5th International Workshop on Adaptive Optics for Industry and Medicine, 2005, Beijing, China

Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

Citation Download Citation

S. P. Poland, D. Burns, W. Lubeigt, B. A. Patterson, G. Valentine, A. J. Wright, and J. M. Girkin "Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy", Proc. SPIE 6018, 5th International Workshop on Adaptive Optics for Industry and Medicine, 60181H (8 June 2006); https://doi.org/10.1117/12.669388

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