Determining biomolecular structures by time-resolved fluorescence

Olaf J. Rolinski; Aaron Hernandez-Santana; Duncan Graham

doi:10.1117/12.710984

27 October 2006 Determining biomolecular structures by time-resolved fluorescence

Olaf J. Rolinski, Aaron Hernandez-Santana, Duncan Graham

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472N (2006) https://doi.org/10.1117/12.710984
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

We demonstrate a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

Citation Download Citation

Olaf J. Rolinski, Aaron Hernandez-Santana, and Duncan Graham "Determining biomolecular structures by time-resolved fluorescence", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472N (27 October 2006); https://doi.org/10.1117/12.710984

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