Two-photon fluorescence anisotropy imaging

Wei Li; Yi Wang; Hanrong Shao; Yonghong He; Hui Ma

doi:10.1117/12.710987

27 October 2006 Two-photon fluorescence anisotropy imaging

Wei Li, Yi Wang, Hanrong Shao, Yonghong He, Hui Ma

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472R (2006) https://doi.org/10.1117/12.710987
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

We have developed a novel method for imaging the fluorescence intensity and anisotropy by two-photon fluorescence microscopy and tested its capability in biological application. This method is applied to model sample including FITC and FITC-CD44 antibody solution and also FITC-CD44 stained cells. The fluorescence anisotropy (FA) of FITC-CD44ab solution is higher than the FITC solution with the same concentration. The fluorescence in cell sample has even higher FA than in solution because the rotation diffusion is restrained in membrane. The method is employed to study the effect of berberine a kind of Chinese medicine, on tumor metastasis. The results indicated that tumor cell membrane fluidity is decreasing with increasing the concentration of berberine in culture medium.

Citation Download Citation

Wei Li, Yi Wang, Hanrong Shao, Yonghong He, and Hui Ma "Two-photon fluorescence anisotropy imaging", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472R (27 October 2006); https://doi.org/10.1117/12.710987

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