Feasibility of chemiluminescence as photodynamic therapy dosimetor

Yanfang Qin; Da Xing; Xueyun Zhong; Jin Zhou; Shiming Luo; Qun Chen

doi:10.1117/12.710705

27 October 2006 Feasibility of chemiluminescence as photodynamic therapy dosimetor

Yanfang Qin, Da Xing, Xueyun Zhong, Jin Zhou, Shiming Luo, Qun Chen

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473B (2006) https://doi.org/10.1117/12.710705
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Photodynamic therapy (PDT) utilizes light energy of a proper wavelength to activate a pre-administered photosensitizer in a target tissue to achieve a localized treatment effect. Current treatment protocol of photodynamic therapy (PDT) is defined by empirical values such as irradiation light fluence, fluence rate and the amount of administered photosensitizer. It is well known that Singlet oxygen is the most important cytotoxic agent responsible for PDT biological effects. An in situ monitoring of singlet oxygen production during PDT would provide a more accurate dosimeter for PDT. The presented study has investigated the feasibility of using Fhioresceinyl Cypridina Luciferin Analog (FCLA), a singlet oxygen specific chemiluminescence (CL) probe, as a dosimetric tool for PDT. Raji lymphoma cell suspensions were sensitized with Photofrin^(R) of various concentrations and irradiated with 635 nm laser light at different fluence rates. FCLA-CL from singlet oxygen produced by the treatment was measured, in real time, with a photon multiplier tube (PMT) system, and linked to the cytotoxicity resulting from the treatment. We have observed that the CL intensity of FCLA is dependent on the PDT treatment parameters. After each PDT treatment and CL measurement, the irradiated cells were evaluated by MIT assay for their Viability. The results show that the cell viability is highly related to the accumulated CL. With ¹0₂ quencher, we confirmed that the CL was mainly related to PDT produced ¹0₂ The results suggest that the FCLA-CL system can be an effective means in measuring PDT ¹O₂ production and may provide an alternative dosimetry technique for PDT.

Citation Download Citation

Yanfang Qin, Da Xing, Xueyun Zhong, Jin Zhou, Shiming Luo, and Qun Chen "Feasibility of chemiluminescence as photodynamic therapy dosimetor", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473B (27 October 2006); https://doi.org/10.1117/12.710705

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KEYWORDS

Photodynamic therapy

Oxygen

Chemiluminescence

Quenching (fluorescence)

Laser therapeutics

Analog electronics

Lymphoma

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