Enhancement mechanism of FCLA-1O2 chemiluminescence by human serum albumin

Jing Zhou; Da Xing; Qun Chen

doi:10.1117/12.710725

27 October 2006 Enhancement mechanism of FCLA-¹O₂ chemiluminescence by human serum albumin

Jing Zhou, Da Xing, Qun Chen

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473G (2006) https://doi.org/10.1117/12.710725
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Fluoresceinyl Cypridina Lucifenn Analog (FCLA) is a reactive oxygen species (ROS) specific chemiluminescence (CL) probe. Its detection efficiency of singlet oxygen (¹0₂)couldbe significantly enhanced in the presence of human serum albumin (HSA). The enhancement mechanism of HSA-FCLA CL is studied in the current work by means ofdirect CL measurement and spectroscopy. The results show that, FCLA can combine with HSA. HSA is an effective ¹O₂ quencher. It can react with ¹0₂ and produce a protein carbonyl group with an elevated energy state. The HSA protein carbonyl group can transfer its energy to FCLA in the FCLA-HSA complex. Via this irradiative de-excitation pathway of the excited FCLA, luminescence production from FCLA is greatly enhanced, in addition to the chemiluminescence from the direct interaction of FCLA and ¹0₂ FCLA has been reported for cancer diagnosis in vivo. Considering HSA is a natural protein that is present in all parts of a human body, the efficacy of FCLA used in vivo is expected to be enhanced through the coupling of FCLA and HSA.

Citation Download Citation

Jing Zhou, Da Xing, and Qun Chen "Enhancement mechanism of FCLA-¹O₂ chemiluminescence by human serum albumin", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473G (27 October 2006); https://doi.org/10.1117/12.710725

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available