6-DOF control of single living cells by proximal two-beam optical tweezers

M. Yoshida; I. Ishimaru; K. Ishizaki; Y. Inoue; T. Yasokawa

doi:10.1117/12.648342

5 December 2005 6-DOF control of single living cells by proximal two-beam optical tweezers

M. Yoshida, I. Ishimaru, K. Ishizaki, Y. Inoue, T. Yasokawa

Proceedings Volume 6048, Optomechatronic Actuators and Manipulation; 604804 (2005) https://doi.org/10.1117/12.648342
Event: Optomechatronic Technologies 2005, 2005, Sapporo, Japan

Abstract

We performed a spectroscopy-tomography study of a single living cell to obtain 3-dimensional distribution of proteins in high spatial resolution in real time. In this report, we mention the 6-DOF manipulation of a single living cell to achieve the high spatial resolution 3-dimentional spectrometry. We propose the proximal two- beam optical tweezers as rotational operation. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can operate the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell. And we propose the optical spatial filtering method, paying attention to the diffracted light that is generated from a sample, as translational velocity measurement. This measurement derives the arbitrary component of the spatial frequency from the random refracted index distribution as the periodic light intensity distribution. This periodic light intensity distribution changes in accordance with the translation of an object. Therefore, we can obtain the translational velocity of the non- labeled cell by high-response photodiode.

Citation Download Citation

M. Yoshida, I. Ishimaru, K. Ishizaki, Y. Inoue, and T. Yasokawa "6-DOF control of single living cells by proximal two-beam optical tweezers", Proc. SPIE 6048, Optomechatronic Actuators and Manipulation, 604804 (5 December 2005); https://doi.org/10.1117/12.648342

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available