In vivo fibered confocal reflectance imaging: totally non-invasive morphological cellular imaging brought to the endoscopist

Anne Osdoit; Magalie Genet; Aymeric Perchant; Sacha Loiseau; Benjamin Abrat; François Lacombe

doi:10.1117/12.646659

23 February 2006 In vivo fibered confocal reflectance imaging: totally non-invasive morphological cellular imaging brought to the endoscopist

Anne Osdoit, Magalie Genet, Aymeric Perchant, Sacha Loiseau, Benjamin Abrat, François Lacombe

Author Affiliations +

Proceedings Volume 6082, Endoscopic Microscopy; 608208 (2006) https://doi.org/10.1117/12.646659
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

This paper presents a novel fibered confocal reflectance microscopy system (FCRM) specifically designed for the medical observation of biological tissues in vivo and in situ, in real time, at the cellular level: the R-600. Reflectance imaging is based on the refraction index difference between biological components while confocal imaging allow to perform the optical sectioning slice in-depth inside the tissues. The R-600 is based on a proximal scanning system, coupled with a 7 mm diameter probe made of tens of thousands of flexible optical fibers allowing in situ imaging, associated with a dedicated software performing real-time control and image processing. The R-600 provides 12 frames per second at an optical imaging depth of 30 microns, with a high lateral resolution, 1 micron, an axial resolution of 2 microns in a field of view 160 microns in diameter. Thanks to the miniaturization of the optical probe, unprecedented accessibility is made possible in organs such as the cervix or the otolaryngological sphere, in a completely non-invasive fashion. The aim of FCRM is to perform optical biopsy. As a first step towards this goal, we present here results obtained in vivo and in real-time on a human mouth , assessing the ability of the R-600 to perform rapid morphologic examination. Subcellular structures such as nuclei and membranes can be clearly distinguished on the images. Further miniaturization opens perspectives for an integrated endoscope-compatible system with broad medical applications.

Citation Download Citation

Anne Osdoit, Magalie Genet, Aymeric Perchant, Sacha Loiseau, Benjamin Abrat, and François Lacombe "In vivo fibered confocal reflectance imaging: totally non-invasive morphological cellular imaging brought to the endoscopist", Proc. SPIE 6082, Endoscopic Microscopy, 608208 (23 February 2006); https://doi.org/10.1117/12.646659

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