Elastic scattering spectroscopy of coagulated brain tissues

Filiz Ateş; Haşim Özgür Tabakoğlu; Özgüncem Bozkulak; Murat Canpolat; Murat Gülsoy

doi:10.1117/12.646195

28 February 2006 Elastic scattering spectroscopy of coagulated brain tissues

Filiz Ateş, Haşim Özgür Tabakoğlu, Özgüncem Bozkulak, Murat Canpolat, Murat Gülsoy

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60840Q (2006) https://doi.org/10.1117/12.646195
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can be referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80 °C) and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

Citation Download Citation

Filiz Ateş, Haşim Özgür Tabakoğlu, Özgüncem Bozkulak, Murat Canpolat, and Murat Gülsoy "Elastic scattering spectroscopy of coagulated brain tissues", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840Q (28 February 2006); https://doi.org/10.1117/12.646195

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KEYWORDS

Tissues

Brain

Scattering

Cerebellum

Light scattering

Spectroscopy

Visible radiation

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