Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo

Takashi Sakurai; Seiji Yamamoto; Atsuo Miyakawa; Yoshihiko Wakazono; Takato O. Yoshida; Eiji Kohno; Remi Tsuchiya-Susuki; Yong Wang; Hideo Hirukawa; Susumu Terakawa

doi:10.1117/12.645939

16 February 2006 Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo

Takashi Sakurai, Seiji Yamamoto, Atsuo Miyakawa, Yoshihiko Wakazono, Takato O. Yoshida, Eiji Kohno, Remi Tsuchiya-Susuki, Yong Wang, Hideo Hirukawa, Susumu Terakawa

Author Affiliations +

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 608803 (2006) https://doi.org/10.1117/12.645939
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

To study cellular morphology and functions in vivo in realtime, we developed a fiber-coupled confocal microscope (FCM), and observed fluorescently-labeled cells inside the body of anesthetized rat. We developed an imaging fiber bundle (IFB), which consisted of an objective lens and a multi-fiber assembly (unit fiber: NA > 0.4, 3 micron in diameter). By combining the IFB with a real-time confocal scanner, we detected intracellular signals of the molecular messenger, and the death signals in the form of fluorescence changes even from cells located deep (> 2 mm) inside the solid organs. The FCM we developed is very promising for detailed studies in both the cell-based researches and clinical researches.

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Takashi Sakurai, Seiji Yamamoto, Atsuo Miyakawa, Yoshihiko Wakazono, Takato O. Yoshida, Eiji Kohno, Remi Tsuchiya-Susuki, Yong Wang, Hideo Hirukawa, and Susumu Terakawa "Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608803 (16 February 2006); https://doi.org/10.1117/12.645939

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