Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope

Shih-hui Chao; Mark R. Holl; Sarah C. McQuaide; Deirdre R. Meldrum

doi:10.1117/12.644939

21 February 2006 Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope

Shih-hui Chao, Mark R. Holl, Sarah C. McQuaide, Deirdre R. Meldrum

Author Affiliations +

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 60880S (2006) https://doi.org/10.1117/12.644939
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A digital light modulation microscope (DLMM) using a digital micro-mirror device (DMD, Texas Instruments) has been developed to enable detection of O₂ concentration in micro-bioreactors using O₂-quenching porphyrin phosphorescent dyes. The emission intensity and phosphorescence lifetime of such dyes are both a function of O₂ concentration. While emission intensity can vary in these dye systems as a function of concentration and illumination intensity, phosphorescence lifetime is primarily sensitive to only O₂ concentration. In contrast to conventional phosphorescence lifetime imaging, the DLMM eliminates the need for a pulsed light source, scanning mirrors, or a high-speed camera for time-gated imaging. This technique can selectively address structured light illumination to each sensor location, which is a beneficial feature for analysis of large micro-sensor arrays within lab-on-a-chip devices. The mirrors on the DMD perform as electronically addressable optical switches, each having a ~15 μs switching time, shorter than the phosphorescence lifetimes of potential O₂ sensing dyes (~25-100 μs). The structured light pattern of the DMD and the switching rate of the mirrors are controlled by a PC. An arc lamp illuminates the DMD uniformly and then projects to the specimen through a filter cube for the selected phosphorescent sensor compound. The emitted light returns to the filter cube and is detected by a photo multiplier tube (PMT). An oscilloscope is used to record the emission signal waveform from the PMT. To demonstrate O₂ sensing with lab-on-a-chip devices, an array of 150-μm-diameter micro-wells coated with phosphorescent porphyrin were observed using the DLMM. The goal of this platform is to measure the O₂ consumption of individual cells trapped in the microwells.

Citation Download Citation

Shih-hui Chao, Mark R. Holl, Sarah C. McQuaide, and Deirdre R. Meldrum "Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880S (21 February 2006); https://doi.org/10.1117/12.644939

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available