Hybrid TiO<sub>2</sub> nanoparticles: an approach for developing site specific DNA cleavage

J. Liu; Z. Saponjic; N. M. Dimitrijevic; S. Luo; D. Preuss; T. Rajh

doi:10.1117/12.661778

27 March 2006 Hybrid TiO₂ nanoparticles: an approach for developing site specific DNA cleavage

J. Liu, Z. Saponjic, N. M. Dimitrijevic, S. Luo, D. Preuss, T. Rajh

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60960F (2006) https://doi.org/10.1117/12.661778
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have developed hybrid light responsive TiO₂ nanoparticles electronically linked to PNA oligonucleotides that site specifically bind to double stranded target DNA. This opens a new opportunity for the development of a highly efficient "artificial restriction enzyme" whose activity can be controlled by using light. The work focuses on the use of TiO₂ nanocomposites as analogs of restriction enzymes with unique specificity that does not exist in current biological approaches. TiO₂ nanoparticles electronically linked to DNA or PNA adapters have been site-specifically attached along double stranded λ DNA vectors. Illumination of this assembly results in selective oxidation of DNA at the deepest "thermodynamic traps" located closest to the nanoparticle surface, causing DNA cleavage. We investigate the effect of the sequence and length of DNA and PNA adapters on the specificity of DNA cleavage. Related to this issue, the potential use of TiO₂/DNA nanocomposites as "rare cutters" that cleave DNA in the places not achieved with existing protein-based enzymes is investigated.

Citation Download Citation

J. Liu, Z. Saponjic, N. M. Dimitrijevic, S. Luo, D. Preuss, and T. Rajh "Hybrid TiO₂ nanoparticles: an approach for developing site specific DNA cleavage", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960F (27 March 2006); https://doi.org/10.1117/12.661778

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