Quantum dot-based fluorescence resonance energy transfer multiplexing: applications for biosensing

Aaron R. Clapp; Igor L. Medintz; Hedi Mattoussi

doi:10.1117/12.647194

27 March 2006 Quantum dot-based fluorescence resonance energy transfer multiplexing: applications for biosensing

Aaron R. Clapp, Igor L. Medintz, Hedi Mattoussi

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60960I (2006) https://doi.org/10.1117/12.647194
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Colloidal quantum dots (QDs) possess numerous physical and optical properties that are ideal for biosensing and multiplexing applications. Fluorescence resonance energy transfer (FRET) is a well-established technique for detecting molecular-scale interactions due to proximity-driven changes in fluorescence. We have shown that QDs are excellent energy donors where dye labeled proteins serve as acceptors, and developed a number of prototype nanosensors for small molecules in solution. Among the more promising potential uses of QD-based FRET nanosensors is the ability to "multiplex" signal channels for parallel detection. Because of their very broad absorption and narrow symmetric emission spectra, QDs are ideal fluorophores for multiplexing applications. In this paper, we describe the benefits of QDs in FRET-based assays (as donors and acceptors) and the potential for signal multiplexing in nanoscale biosensors.

Citation Download Citation

Aaron R. Clapp, Igor L. Medintz, and Hedi Mattoussi "Quantum dot-based fluorescence resonance energy transfer multiplexing: applications for biosensing", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960I (27 March 2006); https://doi.org/10.1117/12.647194

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