Nanosurgery of sub-cellular organelles in living cells using a femtosecond laser oscillator

Wataru Watanabe; Tomoko Shimada; Sachihiro Matsunaga; Hiroshi Ishii; Tsunehito Higashi; Kiichi Fukui; Kazuyoshi Itoh

doi:10.1117/12.645474

28 February 2006 Nanosurgery of sub-cellular organelles in living cells using a femtosecond laser oscillator

Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Hiroshi Ishii, Tsunehito Higashi, Kiichi Fukui, Kazuyoshi Itoh

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Proceedings Volume 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI; 610804 (2006) https://doi.org/10.1117/12.645474
Event: Lasers and Applications in Science and Engineering, 2006, San Jose, California, United States

Abstract

Femtosecond laser pulses in the near-infrared region have potential applications in nanosurgery in cell biology. Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles in living cells. We report on disruption of fluorescence-labeled nucleus and mitochondria in living HeLa cells using a femtosecond Ti:sapphire laser oscillator with a repetition rate of 76 MHz. We examined the effectiveness of the restaining method in combination with fluorescence recovery after photobleaching (FRAP) analysis to discern disruption or bleaching.

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Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Hiroshi Ishii, Tsunehito Higashi, Kiichi Fukui, and Kazuyoshi Itoh "Nanosurgery of sub-cellular organelles in living cells using a femtosecond laser oscillator", Proc. SPIE 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI, 610804 (28 February 2006); https://doi.org/10.1117/12.645474

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KEYWORDS

Femtosecond phenomena

Luminescence

Oscillators

Confocal microscopy

Objectives

Sapphire lasers

Laser therapeutics

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