Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling

J. Podskocova; D. Chorvat Jr.; G. Kollarikova; I. Lacik

doi:10.1117/12.697290

21 July 2006 Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling

J. Podskocova, D. Chorvat Jr., G. Kollarikova, I. Lacik

Author Affiliations +

Proceedings Volume 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII; 61630V (2006) https://doi.org/10.1117/12.697290
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

In this contribution we show application of multispectral confocal laser scanning microscopy for visualisation and characterisation of polyelectrolyte microcapsules. Fluorescent labels of different free charges, specifically bound by electrostatic interactions to the polymer chains in polyelectrolyte microcapsules, were used for questioning the spatial distribution of polymer components of the microcapsules. To separate the overlapping emission spectra of the fluorescent labels spectral imaging and linear unmixing approaches was used.

Citation Download Citation

J. Podskocova, D. Chorvat Jr., G. Kollarikova, and I. Lacik "Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61630V (21 July 2006); https://doi.org/10.1117/12.697290

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available