Cytokine production of the neutrophils and macrophages in time of phagocytosis under influence of infrared low-level laser irradiation

Dmitry V. Rudik; Elena I. Tikhomirova; Elena S. Tuchina

doi:10.1117/12.697429

24 July 2006 Cytokine production of the neutrophils and macrophages in time of phagocytosis under influence of infrared low-level laser irradiation

Dmitry V. Rudik, Elena I. Tikhomirova, Elena S. Tuchina

Author Affiliations +

Proceedings Volume 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII; 61631S (2006) https://doi.org/10.1117/12.697429
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

Influence of infrared low-level laser irradiation (LLLI) on induction of synthesis of some cytokines such as interleykin-1 (Il-1), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleykin-8 (Il-8) and interleykin-4 (Il-4) by the neutrophils and macrophages in time of bacterial cells phagocytosis that was searched. As the object of analysis we used peritoneal macrophages from white mice and neutrophils from peripheral blood of healthy donors. We used the laser diod with spectrum maximum of 850 nm with doses 300, 900 and 1500 mJ (exposition -60, 180 and 300 s respectively; capacity - 5 mW). We carried out the Enzyme-Linked Immunospot Assay (ELISA) to determine cytokine content during phagocytosis after 3 h and 6 h. We found dynamics in production of the cytokines, which was different for the neutrophils and macrophages. We showed that the infrared LLLI has significant stimulating activity on the proinflammatory cytokines production by neutrophils and macrophages. Moreover we revealed dynamics changing in the Il-8 and Il-4 production.

Citation Download Citation

Dmitry V. Rudik, Elena I. Tikhomirova, and Elena S. Tuchina "Cytokine production of the neutrophils and macrophages in time of phagocytosis under influence of infrared low-level laser irradiation", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61631S (24 July 2006); https://doi.org/10.1117/12.697429

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available