Principal component analysis of bacteria using surface-enhanced Raman spectroscopy

Jason Guicheteau; Steven D. Christesen

doi:10.1117/12.664502

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19 May 2006 Principal component analysis of bacteria using surface-enhanced Raman spectroscopy

Jason Guicheteau, Steven D. Christesen

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Proceedings Volume 6218, Chemical and Biological Sensing VII; 62180G (2006) https://doi.org/10.1117/12.664502
Event: Defense and Security Symposium, 2006, Orlando (Kissimmee), Florida, United States

Abstract

Surface-enhanced Raman scattering (SERS) provides rapid fingerprinting of biomaterial in a non-destructive manner. The problem of tissue fluorescence, which can overwhelm a normal Raman signal from biological samples, is largely overcome by treatment of biomaterials with colloidal silver. This work presents a study into the applicability of qualitative SER spectroscopy with principal component analysis (PCA) for the discrimination of four biological threat simulants; Bacillus globigii, Pantoea agglomerans, Brucella noetomae, and Yersinia rohdei. We also demonstrate differentiation of gram-negative and gram-positive species and as well as spores and vegetative cells of Bacillus globigii.

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Jason Guicheteau and Steven D. Christesen "Principal component analysis of bacteria using surface-enhanced Raman spectroscopy", Proc. SPIE 6218, Chemical and Biological Sensing VII, 62180G (19 May 2006); https://doi.org/10.1117/12.664502

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