Detection of <i>Salmonella typhimurium </i>using phage-based magnetostrictive sensor

Ramji S. Lakshmanan; Jing Hu; Rajesh Guntupalli; Jiehui Wan; Shichu Huang; Hong Yang; Valery A. Petrenko; James M. Barbaree; Bryan A. Chin

doi:10.1117/12.665841

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19 May 2006 Detection of Salmonella typhimurium using phage-based magnetostrictive sensor

Ramji S. Lakshmanan, Jing Hu, Rajesh Guntupalli, Jiehui Wan, Shichu Huang, Hong Yang, Valery A. Petrenko, James M. Barbaree, Bryan A. Chin

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Proceedings Volume 6218, Chemical and Biological Sensing VII; 62180Z (2006) https://doi.org/10.1117/12.665841
Event: Defense and Security Symposium, 2006, Orlando (Kissimmee), Florida, United States

Abstract

This article presents a contactless, remote sensing Salmonella typhimurium sensor based on the principle of magnetostriction. Magnetostrictive materials have been used widely for various types of sensor systems. In this work, the use of a magnetostrictive material for the detection of Salmonella typhimurium has been established. The mass of the bacteria attached to the sensor causes changes in the resonance frequency of the sensor. Filamentous bacteriophage was used as a probe order to ensure specific and selective binding of the bacteria onto the sensor surface. Thus changes in response of the sensor due to the mass added onto the sensor caused by specific attachment of bacteria can be monitored in absence of any contact to the sensor. The response of the sensor due to increasing concentrations (from 5x10¹ to 5x10⁸ cfu/ml) of the bacteria was studied. A reduction in the physical dimensions enhances the sensitivity of these sensors and hence different dimensions of the sensor ribbons were studied. For a 2mm x 0.1mm x 0.02mm the detection limit was observed to be of the order of 10⁴ cfu/mL and for a sensor of 1mm x 0.2mm x 0.02mm a reduced detection limit of 10³ cfu/mL was achieved.

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Ramji S. Lakshmanan, Jing Hu, Rajesh Guntupalli, Jiehui Wan, Shichu Huang, Hong Yang, Valery A. Petrenko, James M. Barbaree, and Bryan A. Chin "Detection of Salmonella typhimurium using phage-based magnetostrictive sensor", Proc. SPIE 6218, Chemical and Biological Sensing VII, 62180Z (19 May 2006); https://doi.org/10.1117/12.665841

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