Phage-based magnetostrictive-acoustic microbiosensors for detecting Bacillus anthracis spores

J. Wan; H. Yang; R. S. Lakshmanan; R. Guntupalli; S. Huang; J. Hu; V. A. Petrenko; B. A. Chin

doi:10.1117/12.666205

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18 May 2006 Phage-based magnetostrictive-acoustic microbiosensors for detecting Bacillus anthracis spores

J. Wan, H. Yang, R. S. Lakshmanan, R. Guntupalli, S. Huang, J. Hu, V. A. Petrenko, B. A. Chin

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Proceedings Volume 6223, Micro (MEMS) and Nanotechnologies for Space Applications; 62230E (2006) https://doi.org/10.1117/12.666205
Event: Defense and Security Symposium, 2006, Orlando (Kissimmee), Florida, United States

Abstract

Magnetostrictive particles (MSPs) as biosensor platform have been developed recently. The principle of MSPs as sensor platform is the same as that of other acoustic wave devices, such as quartz crystal microbalance. In this paper, the fabrication, characterization and performance of phage-based MSP biosensors for detecting Bacillus anthracis spores are reported. A commercially available magnetostrictive alloy was utilized to fabricate the sensor platform. The phage was immobilized onto the MSPs using physical adsorption technology. The following performance of the phage-based MSP sensors will be presented: sensitivity, response time, longevity, specificity and binding efficacy. The performance of the sensors at static and dynamic conditions was characterized. The experimental results are confirmed by microscopy photographs. The excellent performance including high sensitivity and rapid response is demonstrated. More importantly, it is experimentally found that the phage-based MSP sensors have a much better longevity than antibody-based sensors.

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J. Wan, H. Yang, R. S. Lakshmanan, R. Guntupalli, S. Huang, J. Hu, V. A. Petrenko, and B. A. Chin "Phage-based magnetostrictive-acoustic microbiosensors for detecting Bacillus anthracis spores", Proc. SPIE 6223, Micro (MEMS) and Nanotechnologies for Space Applications, 62230E (18 May 2006); https://doi.org/10.1117/12.666205

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