3D analysis of bone formation around titanium implants using micro-computed tomography (&#956;CT)

Ricardo Bernhardt; Dieter Scharnweber; Bert Müller; Felix Beckmann; Jürgen Goebbels; John Jansen; Henning Schliephake; Hartmut Worch

doi:10.1117/12.681000

7 September 2006 3D analysis of bone formation around titanium implants using micro-computed tomography (μCT)

Ricardo Bernhardt, Dieter Scharnweber, Bert Müller, Felix Beckmann, Jürgen Goebbels, John Jansen, Henning Schliephake, Hartmut Worch

Author Affiliations +

Proceedings Volume 6318, Developments in X-Ray Tomography V; 631807 (2006) https://doi.org/10.1117/12.681000
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

The quantitative analysis of bone formation around biofunctionalised metallic implants is an important tool for the further development of implants with higher success rates. This is, nowadays, especially important in cases of additional diseases like diabetes or osteoporosis. Micro computed tomography (μCT), as non-destructive technique, offers the possibility for quantitative three-dimensional recording of bone close to the implant's surface with micrometer resolution, which is the range of the relevant bony structures. Within different animal models using cylindrical and screw-shaped Ti6Al4V implants we have compared visualization and quantitative analysis of newly formed bone by the use of synchrotron-radiation-based CT-systems in comparison with histological findings. The SRμCT experiments were performed at the beamline BW 5 (HASYLAB at DESY, Hamburg, Germany) and at the BAMline (BESSY, Berlin, Germany). For the experiments, PMMA-embedded samples were prepared with diameters of about 8 mm, which contain in the center the implant surrounded by the bony tissue. To (locally) quantify the bone formation, models were developed and optimized. The comparison of the results obtained by SRμCT and histology demonstrates the advantages and disadvantages of both approaches, although the bone formation values for the different biofunctionalized implants are identical within the error bars. SRμCT allows the clear identification of fully mineralized bone around the different titanium implants. As hundreds of virtual slices were easily generated for the individual samples, the quantification and interactive bone detection led to conclusions of high precision and statistical relevance. In this way, SRμCT in combination with interactive data analysis is proven to be more significant with respect to classical histology.

Citation Download Citation

Ricardo Bernhardt, Dieter Scharnweber, Bert Müller, Felix Beckmann, Jürgen Goebbels, John Jansen, Henning Schliephake, and Hartmut Worch "3D analysis of bone formation around titanium implants using micro-computed tomography (μCT)", Proc. SPIE 6318, Developments in X-Ray Tomography V, 631807 (7 September 2006); https://doi.org/10.1117/12.681000

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available