Quantitative analysis of mineral content in enamel using laboratory microtomography and microhardness analysis

A. E. M. Vieira; A. C. B. Delbem; K. T. Sassaki; M. L. Cannon; S. R. Stock

doi:10.1117/12.681328

7 September 2006 Quantitative analysis of mineral content in enamel using laboratory microtomography and microhardness analysis

A. E. M. Vieira, A. C. B. Delbem, K. T. Sassaki, M. L. Cannon, S. R. Stock

Author Affiliations +

Proceedings Volume 6318, Developments in X-Ray Tomography V; 631823 (2006) https://doi.org/10.1117/12.681328
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

This study evaluates laboratory microtomography and microhardness analysis for quantifying the mineral content of bovine enamel. Fifty enamel blocks were submitted individually for 5 days to a pH-cycling model at 37°C and remained in the remineralizing solution for 2 days. The blocks were treated twice daily for 1 min with NaF dentifrices (Placebo, 275, 550, 1,100 μg F/g and Crest^(R)) diluted in deionized water. Surface microhardness changes (%SMH) and mineral loss (ΔZ) were then calculated. Laboratory microtomography was also used to measure total mineral lost (LMM). Pearson's correlation (p<0.05) was used to determine the relationship between different methods of analysis and dose-response between treatments. Dentifrice fluoride concentration and %SMH and ΔZ were correlated (p<0.05). There was a positive relationship (p<0.05) when comparing LMM vs. ΔZ; a negative relationship (p<0.05) was found for %SMH vs. LMM and %SMH vs. ΔZ. Therefore, both mineral quantification techniques provide adequate precision for studying the bovine enamel-pH-cycling demineralization/remineralization model.

Citation Download Citation

A. E. M. Vieira, A. C. B. Delbem, K. T. Sassaki, M. L. Cannon, and S. R. Stock "Quantitative analysis of mineral content in enamel using laboratory microtomography and microhardness analysis", Proc. SPIE 6318, Developments in X-Ray Tomography V, 631823 (7 September 2006); https://doi.org/10.1117/12.681328

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