Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening

Dirk U. Näther; Roger Fenske; Reynald Hurteaux; Sandra Majno; S. Desmond Smith

doi:10.1117/12.688419

3 November 2006 Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening

Dirk U. Näther, Roger Fenske, Reynald Hurteaux, Sandra Majno, S. Desmond Smith

Author Affiliations +

Proceedings Volume 6372, Advanced Photon Counting Techniques; 637208 (2006) https://doi.org/10.1117/12.688419
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

A new plate reader (Nanotaurus) has been developed by Edinburgh Instruments that has the principle design features of a confocal microscope and utilises the technique of Time Correlated Single Photon Counting for data acquisition. The advantages of Fluorescence Lifetime Measurements in the nanosecond time scale and analysis methods to recover lifetime parameters are discussed based on experimental data. First working assays using changes of lifetime parameters are presented that clearly demonstrate the advantages of the new instrument for biochemical assays and show strong promise for cell-based assays, by utilising the independence of lifetime parameters from sample volume and concentration.

Citation Download Citation

Dirk U. Näther, Roger Fenske, Reynald Hurteaux, Sandra Majno, and S. Desmond Smith "Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening", Proc. SPIE 6372, Advanced Photon Counting Techniques, 637208 (3 November 2006); https://doi.org/10.1117/12.688419

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