Surface plasmon resonance sensors for detection of survival of motor neuron protein

Margaret Mitropanopoulos; Becki Campanaro; Michael Malone; Jean-Francois Masson; Ronald A. Nieman; Christian L. Lorson; Karl S. Booksh

doi:10.1117/12.686497

25 October 2006 Surface plasmon resonance sensors for detection of survival of motor neuron protein

Margaret Mitropanopoulos, Becki Campanaro, Michael Malone, Jean-Francois Masson, Ronald A. Nieman, Christian L. Lorson, Karl S. Booksh

Author Affiliations +

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638008 (2006) https://doi.org/10.1117/12.686497
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

Surface Plasmon Resonance (SPR) spectroscopy is a potentially valuable tool for measuring protein-protein interactions and protein levels in vitro and in vivo. Fiber-optic based SPR allows for rapid quantitative measurement of disease markers such as the truncated (exons 1-7) survival of motor neuron (SMN) protein. Unlike micro fluidics-based SPR systems, sample loss is eliminated in fiber-optic SPR and the small size of the fiber optic probes (400μm or smaller) facilitates the potential for use in vivo. Recombinant SMN protein overexpressed in E. Coli as well as native SMN from cultured HeLa cells has been successfully measured using fiber-optic SPR.

Citation Download Citation

Margaret Mitropanopoulos, Becki Campanaro, Michael Malone, Jean-Francois Masson, Ronald A. Nieman, Christian L. Lorson, and Karl S. Booksh "Surface plasmon resonance sensors for detection of survival of motor neuron protein", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638008 (25 October 2006); https://doi.org/10.1117/12.686497

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