Raman molecular imaging of tissue and cell samples using tunable multiconjugate filter

John Maier M.D.; Janice Panza; Amy Drauch; Shona Stewart

doi:10.1117/12.686243

23 October 2006 Raman molecular imaging of tissue and cell samples using tunable multiconjugate filter

John Maier M.D., Janice Panza, Amy Drauch, Shona Stewart

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638009 (2006) https://doi.org/10.1117/12.686243
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

Raman spectroscopy is a powerful technique for rapid, non-invasive and reagentless analysis of materials, including biological cells and tissues. Raman Molecular Imaging combines high molecular information content Raman spectroscopy and digital full field imaging to enable the investigation of cells and tissues. We have conducted widefield imaging using a new class of birefringent liquid crystal tunable filter that provides high throughput over an extended wavelength range. This tool has been applied to investigate the linkage between reagentless spectral imaging in tissue and cells and standard reagent based approaches. In this report, we describe Raman imaging data on a clinical tissue sample and cultured cells. The results demonstrate the sensitivity of Raman Molecular Imaging and fluorescence spectral imaging to molecular differences in biological systems laying the foundation for the eventual use of this approach as a biological research and clinical diagnostic tool.

Citation Download Citation

John Maier M.D., Janice Panza, Amy Drauch, and Shona Stewart "Raman molecular imaging of tissue and cell samples using tunable multiconjugate filter", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638009 (23 October 2006); https://doi.org/10.1117/12.686243

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