Scaling mass sensitivity of the BioCD at 0.25 pg/mm

David D. Nolte; Ming Zhao

doi:10.1117/12.686307

23 October 2006 Scaling mass sensitivity of the BioCD at 0.25 pg/mm

David D. Nolte, Ming Zhao

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 63800J (2006) https://doi.org/10.1117/12.686307
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

The BioCD is a class of self-referencing interferometric optical biosensor that measures phase modulation from proteins on a spinning disk. The optical detection of the patterns at high speed yields low noise floors far from 1/f noise. Two scans of a disk before and after a 20 hour buffer wash are differenced yielding an rms surface height measurement error of 45 pm corresponding to 5 femtograms of protein within a focal spot diameter. Simple area scaling relations are derived that predict the performance of immunoassays as a function of well area. The scaling mass sensitivity of the BioCD is determined to be 0.25 pg/mm under the conditions of an assay, with a metrology limit of the technique between 0.05 to 0.1 pg/mm. The BioCD sensitivity is equivalent to the best reported surface mass sensitivity of surface plasmon resonance sensors, and is achieved without resonant structures and hence is easy to fabricate and operate.

Citation Download Citation

David D. Nolte and Ming Zhao "Scaling mass sensitivity of the BioCD at 0.25 pg/mm", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800J (23 October 2006); https://doi.org/10.1117/12.686307

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