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1 November 2007 Fluorescence resonance energy transfer between CdTe semiconductor nanocrystals donors and gold nanoparticles labeled DNA acceptors
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Proceedings Volume 6423, International Conference on Smart Materials and Nanotechnology in Engineering; 642357 (2007) https://doi.org/10.1117/12.780662
Event: International Conference on Smart Materials and Nanotechnology in Engineering, 2007, Harbin, China
Abstract
CdTe semiconductor nanocrystals with 2.5 nm in diameters, as energy donors, were prepared in water. Narrow-disperse Au nanoparticles with 16 nm in diameters, as energy acceptors, were prepared from gold chloride by reduction. It was confirmed that the emission spectrum of CdTe ODs and the absorption spectrum of AuNPs had an appreciable overlap. Therefore, a DNA fluorescence probe system based on fluorescence resonance energy transfer (FRET) from CdTe quantum dots donors to gold nanoparticles acceptors was designed. CdTe QDs were linked to 5'-NH2-DNA through 1- ethyl-3-(dimethylaminopr opyl) carbodi-imide hydrochloride (EDC) as a linker, and the 3'-SH-DNA was self-assembled onto the surface of Au nanoparticles. The hybridization of complementary DNA bound to the QDs and Au nanoparticles (CdTe-DNA-Au) determined the FRET distance of CdTe QDs and Au nanoparticles. Compared with the fluorescence of CdTe-DNA, the fluorescence of CdTe-DNA-Au conjugates decreased extremely, which indicated that the FRET occurred between CdTe ODs and Au nanoparticles. The fluorescence change of this conjugates was depended on the ratio of Au-DNA and CdTe-DNA, when the AuNPs-DNA to QD-DNA ratio is 5:1, the FRET efficiency reached a maximum. If a complementary single stranded DNA was add to the CdTe-DNA-Au conjugates, the fluorescence of the probe system would have a certain degree recovery, this showed that the distance between CdTe QDs and Au nanoparticles was increased.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zhao Dai, Jimei Zhang, Shichao Xu, Ning Guo, Yuehua Bu, and Quanxi Dong "Fluorescence resonance energy transfer between CdTe semiconductor nanocrystals donors and gold nanoparticles labeled DNA acceptors", Proc. SPIE 6423, International Conference on Smart Materials and Nanotechnology in Engineering, 642357 (1 November 2007); https://doi.org/10.1117/12.780662
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