Multiphoton microscopy of atheroslcerotic plaques

Magnus B. Lilledahl; Catharina de Lange Davies; Olav A. Haugen; Lars O. Svaasand

doi:10.1117/12.706469

23 March 2007 Multiphoton microscopy of atheroslcerotic plaques

Magnus B. Lilledahl, Catharina de Lange Davies, Olav A. Haugen, Lars O. Svaasand

Proceedings Volume 6424, Photonic Therapeutics and Diagnostics III; 64241Y (2007) https://doi.org/10.1117/12.706469
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Multiphoton microscopy is a techniques that fascilitates three dimensional imaging of intact, unstained tissue. Especially connective tissue has a relatively strong nonlinear optical response and can easily be imaged. Atherosclerosis is a disease where lipids accumulate in the vessel wall and there is a thickening of the intima by growth of a cap of connective tissue. The mechanical strength of this fibrous cap is of clinically importance. If the cap ruptures a thrombosis forms which can block a coronary vessel and therby causing myocardial infarction. Multiphoton microscopy can be used to image the fibrous cap and thereby determine the thickness of the cap and the structure of the connective fibres. This could possibly be developed into a diagnostic and clincal tool to monitor the vulnerability of a plaque and also to better understand the development of a plaque and effects of treatment. We have collected multiphoton microscopy images from atherosclerotic plaque in human aorta, both two photon excited fluorescens and second harmonic generated signal. The feasability of using this technique to determine the state of the plaque is explored.

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Magnus B. Lilledahl, Catharina de Lange Davies, Olav A. Haugen, and Lars O. Svaasand "Multiphoton microscopy of atheroslcerotic plaques", Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64241Y (23 March 2007); https://doi.org/10.1117/12.706469

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KEYWORDS

Collagen

Second-harmonic generation

Multiphoton microscopy

Tissues

Arteries

Connective tissue

Image resolution

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