X-ray and optical multimodality tomographer for small animal examination

A. Da Silva; M. Leabad; T. Bordy; J.-M. Dinten; P. Peltié; P. Rizo

doi:10.1117/12.699863

8 February 2007 X-ray and optical multimodality tomographer for small animal examination

A. Da Silva, M. Leabad, T. Bordy, J.-M. Dinten, P. Peltié, P. Rizo

Proceedings Volume 6431, Multimodal Biomedical Imaging II; 64310R (2007) https://doi.org/10.1117/12.699863
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A small animal multimodality tomographer dedicated to the co-registration of fluorescence optical signal and X-rays measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility to get in vivo anatomical and functional information at once. Moreover, anatomical measurements can be used as a regularization factor in order to get the reconstructions of the biodistribution of fluorochromes more accurate and to speed up the treatment. The optical system is basically composed with a CW laser (Krypton, 752 nm) for an optimal excitation of Alexa-Fluor 750 fluorochromes, and a CCD camera coupled with a combination of filters for the fluorescence detection. The animal is placed inside a transparent tube filled with an index matching fluid. In order to perform multiple views of fluorescence data acquisitions, the cylinder is fixed to a rotating stage. The excitation beam is brought to the cylinder via two mirrors mounted on translation plates allowing a vertical scan. The optical data acquisitions are performed with a high sensitivity CCD camera. The X-ray generator and the X-ray detector have been placed perpendicularly to the optical chain. A first study on phantoms was conducted to evaluate the feasibility, to test the linearity and the reproducibility, and to fix the parameters for the co-registration. These test experiments were reproduced by considering mice in the oesophagus of which the previous tubes were inserted. Finally, the performance of the system was evaluated in vivo on mice bearing tumours in the lungs, tagged with Transferrin-AlexaFluor 750.

Citation Download Citation

A. Da Silva, M. Leabad, T. Bordy, J.-M. Dinten, P. Peltié, and P. Rizo "X-ray and optical multimodality tomographer for small animal examination", Proc. SPIE 6431, Multimodal Biomedical Imaging II, 64310R (8 February 2007); https://doi.org/10.1117/12.699863

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

X-ray optics

Inverse optics

Luminescence

X-rays

Data acquisition

Geometrical optics

In vivo imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years