Experimental evaluation of angularly variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms

Adrien Ming Jer Wang; Vengadesan Nammalvar; Rebekah Anna Drezek

doi:10.1117/12.702005

14 February 2007 Experimental evaluation of angularly variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms

Adrien Ming Jer Wang, Vengadesan Nammalvar, Rebekah Anna Drezek

Author Affiliations +

Proceedings Volume 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII; 64330B (2007) https://doi.org/10.1117/12.702005
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The aim of the present study focuses on experimentally demonstrating the efficacy of using angularly-variable fiber geometry to achieve the desired tissue-layer selection and probing depths with the further objective of enhancing the sensitivity and specificity of spectral diagnosis in stratified architectures that resemble human cervical epithelia. The morphological and biochemical features of epithelial tissue vary in accordance with tissue depths; consequently, the accuracy of spectroscopic diagnosis of epithelial dysplasia may be enhanced by probing the optical properties of this tissue. When correlated to cellular dysplasia, layer-specific changes in tissue optical properties may be deciphered by reflectance spectroscopy coupled with angularly-variable fiber geometry. This study addresses the utility of using such angularly-variable fiber geometry for resolving spatially-specific spectral signatures of tissue pathology. This is accomplished by interpreting and analyzing the reflectance spectra of increasingly dysplastic epithelial tissue in two-layer epithelial phantoms. Spectral sensitivity to tissue abnormalities in the epithelial layer is significantly improved as the obliquity of the collection fibers increases from 0 to 40 degrees. Conversely, conventionally orthogonal fibers are found to be more sensitive to changes in stromal tissue properties.

Citation Download Citation

Adrien Ming Jer Wang, Vengadesan Nammalvar, and Rebekah Anna Drezek "Experimental evaluation of angularly variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms", Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330B (14 February 2007); https://doi.org/10.1117/12.702005

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available