New components for fiber-optic thin-layer chromatography including fluorescence

Michaela Bleichert; Hanns Simon Eckhardt; Karl-Friedrich Klein; Bernd Spangenberg; Georg Hillrichs; Joachim Mannhardt

doi:10.1117/12.717579

15 February 2007 New components for fiber-optic thin-layer chromatography including fluorescence

Michaela Bleichert, Hanns Simon Eckhardt, Karl-Friedrich Klein, Bernd Spangenberg, Georg Hillrichs, Joachim Mannhardt

Proceedings Volume 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII; 64330F (2007) https://doi.org/10.1117/12.717579
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In thin-layer chromatography, fiber-bundle arrays have been introduced for spectral absorption measurements in the UV-region. Using all-silica fiber bundles, the exciting light will be detected after re-emission on the plate with a fiberoptic spectrometer. In addition, fluorescence light can be detected which will be masked by the re-emitted light. Therefore, it is helpful to separate the absorption and fluorescence on the TLC-plate. A modified three-array assembly has been developed: using one array for detection, the two others are used for excitation with broadband band deuterium-light and with UV-LEDs adjusted to the substances under test. As an example, the quantification of glucosamine in nutritional supplements or spinach leaf extract will be described. Using simply heating of the amino-plate for derivation, the reaction product of Glucosamine can be detected sensitively either by light absorption or by fluorescence, using the new fiber-optic assembly. In addition, the properties of the new 3-row fiber-optic array and the commercially available UV-LEDs will be shown, in the interesting wavelength region for excitation of fluorescence, from 260 nm to 360 nm. The squint angle having an influence on coupling efficiency and spatial resolution will be measured with the inverse farfield method. Some properties of UV-LEDs for analytical applications will be described and discussed, too.

Citation Download Citation

Michaela Bleichert, Hanns Simon Eckhardt, Karl-Friedrich Klein, Bernd Spangenberg, Georg Hillrichs, and Joachim Mannhardt "New components for fiber-optic thin-layer chromatography including fluorescence", Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330F (15 February 2007); https://doi.org/10.1117/12.717579

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