Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography: laser induced fluorescence

N. Sujatha; Lavanya Rai; Pratap Kumar; B. R. Krishnanand; K. K. Mahato; Sajan D. George; V. B. Kartha; Santhosh C

doi:10.1117/12.699711

19 February 2007 Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography: laser induced fluorescence

N. Sujatha, Lavanya Rai, Pratap Kumar, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, Santhosh C

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Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410B (2007) https://doi.org/10.1117/12.699711
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

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N. Sujatha, Lavanya Rai, Pratap Kumar, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, and Santhosh C "Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography: laser induced fluorescence", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410B (19 February 2007); https://doi.org/10.1117/12.699711

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