Improved image-forming optics for transmission optical projection tomography

Yi Wang; Ruikang Wang

doi:10.1117/12.702250

14 February 2007 Improved image-forming optics for transmission optical projection tomography

Yi Wang, Ruikang Wang

Author Affiliations +

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 644304 (2007) https://doi.org/10.1117/12.702250
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We demonstrate an improved image-forming optics for transmission optical projection tomography (TOPT), with which the parallel integral throughout an object can be obtained. This is performed by introducing a shutter with an appropriate diameter at the back focus of the objective lens. We evaluate and compare the performances of the improved and the conventional TOPT systems with different parameters to obtain the optimal configuration. The optimal reconstruction is achieved by the improved configuration with α =0.8° ~1.6°, and the spatial resolution reaches 25 μm. The Optimal configuration is validated by TOPT of a phantom sample and a five days chick embryo.

Citation Download Citation

Yi Wang and Ruikang Wang "Improved image-forming optics for transmission optical projection tomography", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644304 (14 February 2007); https://doi.org/10.1117/12.702250

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