An optical technique for fast focusing applied to high-aperture microscopy

Edward Botcherby; Rimas Juskaitis; Martin Booth; Tony Wilson

doi:10.1117/12.705545

14 February 2007 An optical technique for fast focusing applied to high-aperture microscopy

Edward Botcherby, Rimas Juskaitis, Martin Booth, Tony Wilson

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430H (2007) https://doi.org/10.1117/12.705545
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this paper we describe a simple method of optical refocusing for high numerical aperture imaging systems. As the first stage of this new method satisfies both the sine and Herschel conditions of geometric optics, images obtained are free from spherical aberration over a large scan range.

Citation Download Citation

Edward Botcherby, Rimas Juskaitis, Martin Booth, and Tony Wilson "An optical technique for fast focusing applied to high-aperture microscopy", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430H (14 February 2007); https://doi.org/10.1117/12.705545

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