High-speed line scanning confocal microscope for biological imaging

Seung-Hwan Jung; Chang-Keun Kim; Sung-Bin Ju; Yong-Jin Cho; Hyun-Woo Jeong; Beop-Min Kim

doi:10.1117/12.700460

14 February 2007 High-speed line scanning confocal microscope for biological imaging

Seung-Hwan Jung, Chang-Keun Kim, Sung-Bin Ju, Yong-Jin Cho, Hyun-Woo Jeong, Beop-Min Kim

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 644318 (2007) https://doi.org/10.1117/12.700460
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image). This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D structures rapidly.

Citation Download Citation

Seung-Hwan Jung, Chang-Keun Kim, Sung-Bin Ju, Yong-Jin Cho, Hyun-Woo Jeong, and Beop-Min Kim "High-speed line scanning confocal microscope for biological imaging", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644318 (14 February 2007); https://doi.org/10.1117/12.700460

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Confocal microscopy

Microscopes

CCD cameras

Image acquisition

Imaging systems

Objectives

Cornea

Show All Keywords

Keywords/Phrases

Search In:

Publication Years