Luminescent lanthanide-ion doped nanoparticles as single-biomolecule labels and oxidant sensors

D. Casanova; D. Giaume; M. Moreau; T. Gacoin; J. P. Boilot; A. Alexandrou D.D.S.

doi:10.1117/12.705067

Translator Disclaimer

27 February 2007 Luminescent lanthanide-ion doped nanoparticles as single-biomolecule labels and oxidant sensors

D. Casanova, D. Giaume, M. Moreau, T. Gacoin, J. P. Boilot, A. Alexandrou D.D.S.

Author Affiliations +

Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 64480F (2007) https://doi.org/10.1117/12.705067
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We report on the single-particle properties of lanthanide-ion doped oxide nanoparticles. We have demonstrated that their size can be accurately determined from their luminosity. The optically determined size distribution is in very good agreement with the distribution obtained from transmission electron microscopy (TEM). We also showed that the photobleaching of these nanoparticles is related to a reduction process and that we can use it to sense in a concentration-dependent manner the presence of an oxidant like H₂O₂. Finally, we propose a way to perform nanoparticle-protein coupling and to determine the protein-nanoparticle ratio at the single-particle level.

Citation Download Citation

D. Casanova, D. Giaume, M. Moreau, T. Gacoin, J. P. Boilot, and A. Alexandrou D.D.S. "Luminescent lanthanide-ion doped nanoparticles as single-biomolecule labels and oxidant sensors", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480F (27 February 2007); https://doi.org/10.1117/12.705067

ACCESS THE FULL ARTICLE