Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples

Thomas Pons; Igor L. Medintz; Xiang Wang; Douglas S. English; Hedi Mattoussi

doi:10.1117/12.717539

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27 February 2007 Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples

Thomas Pons, Igor L. Medintz, Xiang Wang, Douglas S. English, Hedi Mattoussi

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Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 64480I (2007) https://doi.org/10.1117/12.717539
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We present a single QD FRET study that allows us to probe the heterogeneity in QD-dye labeled protein conjugates. We first show that QDs are compatible with spFRET detection by demonstrating the equivalence of single particle and ensemble measurement modalities in terms of derived average FRET efficiencies and separation distances between a QD donor and dyes attached to specific sites on conjugated proteins. We then use spFRET data to demonstrate that the valence distribution of QD-protein conjugates follows Poisson statistics.

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Thomas Pons, Igor L. Medintz, Xiang Wang, Douglas S. English, and Hedi Mattoussi "Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480I (27 February 2007); https://doi.org/10.1117/12.717539

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