Multiplexed immunoassays for biomonitoring of exposure to agrochemicals using quantum dots as fluorescent reporters

Mikaela Nichkova; Dosi Dosev; Alexander E. Davies; Shirley J. Gee; Ian M. Kennedy; Bruce D. Hammock

doi:10.1117/12.699231

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6 March 2007 Multiplexed immunoassays for biomonitoring of exposure to agrochemicals using quantum dots as fluorescent reporters

Mikaela Nichkova, Dosi Dosev, Alexander E. Davies, Shirley J. Gee, Ian M. Kennedy, Bruce D. Hammock

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Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 644817 (2007) https://doi.org/10.1117/12.699231
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3- phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using quantum dots (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.

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Mikaela Nichkova, Dosi Dosev, Alexander E. Davies, Shirley J. Gee, Ian M. Kennedy, and Bruce D. Hammock "Multiplexed immunoassays for biomonitoring of exposure to agrochemicals using quantum dots as fluorescent reporters", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 644817 (6 March 2007); https://doi.org/10.1117/12.699231

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