Integrated biophotonic hybridization sensor based on chitosan-mediated assembly

Vlad Badilita; Michael Powers; Stephan Koev; Hyunmin Yi; Gregory Payne; Reza Ghodssi

doi:10.1117/12.705612

12 February 2007 Integrated biophotonic hybridization sensor based on chitosan-mediated assembly

Vlad Badilita, Michael Powers, Stephan Koev, Hyunmin Yi, Gregory Payne, Reza Ghodssi

Proceedings Volume 6464, MEMS/MOEMS Components and Their Applications IV; 646404 (2007) https://doi.org/10.1117/12.705612
Event: MOEMS-MEMS 2007 Micro and Nanofabrication, 2007, San Jose, California, United States

Abstract

We present the use of the polysaccharide chitosan for immobilizing biomolecules on microfabricated device surfaces. The main advantages of chitosan are its abundance of primary amine groups and its ability to be electrodeposited. Biomolecules are easily attached to chitosan's amines by standard glutaraldehyde chemistry. The electrodeposition of chitosan allows accurate spatial and temporal control of biomolecule placement. We have used this biofunctionalization approach to develop a biophotonic hybridization sensor. Here we present for the first time probe DNA functionalization of the chitosan interface and hybridization detection using fluorescently labeled target DNA and integrated optical waveguides.

Citation Download Citation

Vlad Badilita, Michael Powers, Stephan Koev, Hyunmin Yi, Gregory Payne, and Reza Ghodssi "Integrated biophotonic hybridization sensor based on chitosan-mediated assembly", Proc. SPIE 6464, MEMS/MOEMS Components and Their Applications IV, 646404 (12 February 2007); https://doi.org/10.1117/12.705612

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